Leuconostoc holzapfelii sp. nov., isolated from Ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of Leuconostoc species

Author:

De Bruyne Katrien1,Schillinger Ulrich2,Caroline Lily2,Boehringer Benjamin2,Cleenwerck Ilse3,Vancanneyt Marc3,De Vuyst Luc4,Franz Charles M. A. P.2,Vandamme Peter1

Affiliation:

1. Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium

2. Federal Research Centre for Nutrition and Food, Institute of Hygiene and Toxicology, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany

3. BCCM/LMG Bacteria Collection, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium

4. Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Department of Applied Biological Sciences and Engineering, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium

Abstract

A Gram-positive, ovoid lactic acid bacterium, strain LMG 23990T, was isolated from Ethiopian coffee fermentation. 16S rRNA gene sequence analysis indicated that the novel strain belongs to the genus Leuconostoc, with Leuconostoc citreum and Leuconostoc lactis as the closest neighbours (99.6 and 99.0 % 16S rRNA gene sequence similarity, respectively). Genotypic fingerprinting by fluorescent amplified fragment length polymorphism, whole-cell protein electrophoresis, DNA–DNA hybridizations, comparative sequence analysis of pheS, rpoA, atpA, and physiological and biochemical tests allowed us to differentiate strain LMG 23990T from all established Leuconostoc species. Strain LMG 23990T (=CCUG 54536T) therefore represents a novel species, for which the name Leuconostoc holzapfelii sp. nov. is proposed.

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

Reference36 articles.

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