Generation of a neutralizing antibody against RD114-pseudotyped viral vectors

Author:

Mekkaoui L.1ORCID,Ferrari M.2,Mattiuzzo G.3ORCID,Ma B.2,Nannini F.1,Onuoha S.2,Kotsopoulou E.2,Takeuchi Y.43,Pule M.

Affiliation:

1. UCL Cancer Institute, University College London, Paul O’Gorman Building, 72 Huntley Street, WC1E 6BT, London, UK

2. Autolus Limited, Forest House, 58 Wood Lane, W12 7RZ, UK

3. National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, EN6 3QC, UK

4. Division of Infection and Immunity, University College London, Cruciform Building, Gower Street, WC1E 6BT, UK

Abstract

The feline endogenous RD114 glycoprotein has proved to be an attractive envelope to pseudotype both retroviral and lentiviral vectors. As a surface protein, its detection on packaging cells as well as viral particles would be useful in different fields of its use. To address this, we generated a monoclonal antibody against RD114 by immunization of rats, termed 22F10. Once seroconversion was confirmed, purified 22F10 was cloned into murine Fc and characterized with a binding affinity of 10nM. The antibody was used to detect RD114 and its variant envelopes on different stable viral packaging cell lines (FLYRD18 and WinPac-RD). 22F10 was also shown to prevent the infections of different strains of RD-pseudotyped vectors but not related envelope glycoproteins by blocking cell surface receptor binding. We are the first to report the neutralization of viral particles by a monoclonal αRD114 antibody.

Publisher

Microbiology Society

Subject

Virology

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