Brevundimonas kwangchunensis sp. nov., isolated from an alkaline soil in Korea

Abstract

Two Gram-negative, rod-shaped bacterial strains, KSL-102Tand KSL-110, were isolated from an alkaline soil in Korea, and their taxonomic positions were investigated by use of a polyphasic study. The two strains grew optimally at pH 7·0–8·0 and 30 °C without NaCl. They contained Q-10 as the predominant ubiquinone. The major fatty acids were C18 : 1ω7cand C16 : 0on trypticase soy agar, but 11-methyl C18 : 1ω7cwas also a major component when the two strains were cultivated on LMG medium no. 221. Their DNA G+C contents were 68·4–68·7 mol%. Strains KSL-102Tand KSL-110 exhibited three nucleotide differences in their 16S rRNA gene sequences and a mean DNA–DNA relatedness value of 85 %. Phylogenetic trees based on 16S rRNA gene sequences showed that the two strains fell within the evolutionary radiation encompassed by the genusBrevundimonas. Levels of 16S rRNA gene sequence similarity between the two strains and the type strains of recognizedBrevundimonasspecies ranged from 96·3 to 98·4 %. DNA–DNA relatedness levels between the two strains and recognizedBrevundimonasspecies were 8–21 %. On the basis of phenotypic, phylogenetic and genetic data, strains KSL-102Tand KSL-110 were classified in the genusBrevundimonasas members of a novel species, for which the nameBrevundimonas kwangchunensissp. nov. is proposed. The type strain is KSL-102T(=KCTC 12380T=DSM 17033T).