Dyella koreensis sp. nov., a β-glucosidase-producing bacterium

Abstract

A bacterial strain (designated BB4T), which has β-glucosidase activity, was isolated from soil around the roots of bamboo plants. Cells were Gram-negative, aerobic, non-motile and straight-rod-shaped. Phylogenetic analysis of 16S rRNA gene sequences revealed a clear affiliation with members of the family ‘Xanthomonadaceae’. The 16S rRNA gene sequence of strain BB4T showed the following sequence similarities: 97·7 % to Dyella japonica XD53T, 97·1 % to Frateuria aurantia LMG 1558T, 96·2 % to Fulvimonas soli LMG 19981T, 94·3 % to Rhodanobacter lindaniclasticus RP5575T and <90 % to other members of the ‘Gammaproteobacteria’. The G+C content of the genomic DNA was 63·8 mol%. The major fatty acids were branched forms, especially large proportions of iso-C15 : 0, iso-C17 : 0 and iso-C17 : 1 ω9c, similar to the profile of the genus Dyella. The results of DNA–DNA hybridization with D. japonica XD53T and Frateuria aurantia LMG 1558T, in combination with phenotypic characteristics and 16S rRNA gene sequence analysis, demonstrated that strain BB4T should be classified as a novel Dyella species. The name Dyella koreensis sp. nov. is proposed, with strain BB4T (=KCTC 12359T=NBRC 100831T) as the type strain.