Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia

Abstract

Several orange- and yellow-pigmented, halophilic, strictly aerobic, chemoheterotrophic, Gram-negative strains were isolated during investigations of maritime Antarctic habitats, including coastal fast sea-ice brine and algae, crustaceans and quartz stone sublithic cyanobacterial biofilms. Isolates investigated in this study belonged to the marine clade of the family Flavobacteriaceae and represented lineages that were either distinct from species with validly published names or appeared to be distinct species within existing genera. A polyphasic taxonomic analysis demonstrated the novelty of these strains, and several new taxa are proposed. Strains from quartz stone sublithic communities were grouped into two new genera designated Subsaximicrobium gen. nov. and Subsaxibacter gen. nov. The genus Subsaximicrobium included the species Subsaximicrobium wynnwilliamsii sp. nov. (type species; type strain G#7T=ACAM 1070T=CIP 108525T) and Subsaximicrobium saxinquilinus sp. nov. (type strain Y4-5T=ACAM 1063T=CIP 108526T). The genus Subsaxibacter contained a single species designated Subsaxibacter broadyi sp. nov. (type strain P7T=ACAM 1064T=CIP 108527T). A novel bacterial strain isolated from the lake-dwelling, calanoid copepod Paralabidocera antarctica was given the name Lacinutrix copepodicola gen. nov., sp. nov. (type strain DJ3T=ACAM 1055T=CIP 108538T). Four novel species of the genus Bizionia were discovered, Bizionia algoritergicola sp. nov. (type strain APA-1T=ACAM 1056T=CIP 108533T) and Bizionia myxarmorum sp. nov. (type strain ADA-4T=ACAM 1058T=CIP 108535T), which were isolated from the carapace surfaces of sea-ice algae-feeding amphipods, and Bizionia gelidisalsuginis sp. nov. (type strain IC164T=ACAM 1057T=CIP 108536T) and Bizionia saleffrena sp. nov. (type strain HFDT=ACAM 1059T=CIP 108534T), which were isolated from sea-ice brines. Several other novel species were also isolated from sea-ice samples, including two novel species of the genus Gelidibacter, Gelidibacter gilvus sp. nov. (type strain IC158T=ACAM 1054T=CIP 108531T) and Gelidibacter salicanalis sp. nov. (type strain IC162T=ACAM 1053T=CIP 108532T), as well as three novel species of the genus Gillisia, Gillisia illustrilutea sp. nov. (type strain IC157T=ACAM 1062T=CIP 108530T), Gillisia sandarakina sp. nov. (type strain IC148T=ACAM 1060T=CIP 108529T) and Gillisia hiemivivida sp. nov. (type strain IC154T=ACAM 1061T=CIP 108528T).