Treponema berlinense sp. nov. and Treponema porcinum sp. nov., novel spirochaetes isolated from porcine faeces

Abstract

Limit-dilution procedures were used to isolate seven, helically coiled bacterial strains from faeces of swine that constituted two unidentified taxa. Comparative 16S rRNA gene sequence analysis showed highest similarity values with species of the genusTreponemaindicating that the isolates are members of this genus. Strain 7CPL208T, as well as five further isolates, and 14V28Tdisplayed the highest 16S rRNA gene sequence similarities withTreponema pectinovorumATCC 33768T(92·3 %) andTreponema parvumOMZ 833T(89·9 %), respectively. Polar lipid profiles distinguished 7CPL208Tand 14V28Tfrom each other as well as from related species. Based on their phenotypic and genotypic distinctiveness, strains 7CPL208Tand 14V28Tare suggested to represent two novel species of the genusTreponema, for which the namesTreponema berlinensesp. nov. andTreponema porcinumsp. nov. are proposed. The type strain forTreponema berlinenseis 7CPL208T(=ATCC BAA-909T=CIP 108244T=JCM 12341T) and forTreponema porcinum14V28T(=ATCC BAA-908T=CIP 108245T=JCM 12342T).