Cellulomonas terrae sp. nov., a cellulolytic and xylanolytic bacterium isolated from soil

Abstract

A bacterial strain (DB5T), with polysaccharide-degrading activities, was isolated from garden soil in Daejeon, Republic of Korea. The cells were Gram-positive, aerobic or facultatively anaerobic, non-motile straight rods. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genusCellulomonasand that it is most closely related toCellulomonas xylanilyticaLMG 21723TandCellulomonas humilataATCC 25174T(98·0 and 97·9 % similarity, respectively). Chemotaxonomic data also supported the classification of strain DB5Tin the genusCellulomonas, i.e.l-ornithine as the cell-wall diamino acid, anteiso-C15 : 0and iso-C15 : 0as the major fatty acids, MK-9(H4) as the predominant menaquinone and the presence of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol mannosides in the polar lipid profile. The results of DNA–DNA hybridization in combination with chemotaxonomic and physiological data demonstrated that strain DB5T(=KCTC 19081T=NBRC 100819T) should be classified as the type strain of a novel species within the genusCellulomonas, for which the nameCellulomonas terraesp. nov. is proposed.