Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein-Reference-Cited by-同舟云学术

Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein

Published:2005-08-01 Issue:8 Volume:86 Page:2269-2274
ISSN:0022-1317
Container-title:Journal of General Virology
language:en
Short-container-title:

Author:

Fukushi Shuetsu¹,Mizutani Tetsuya¹,Saijo Masayuki¹,Matsuyama Shutoku²,Miyajima Naoko²,Taguchi Fumihiro²,Itamura Shigeyuki³,Kurane Ichiro¹,Morikawa Shigeru¹

Affiliation:

1. Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan

2. Laboratory of Respiratory Viral Diseases and SARS, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan

3. Laboratory of Influenza Virus, Department of Virology III, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) contains a single spike (S) protein, which binds to its receptor, angiotensin-converting enzyme 2 (ACE2), induces membrane fusion and serves as a neutralizing antigen. A SARS-CoV-S protein-bearing vesicular stomatitis virus (VSV) pseudotype using the VSVΔG* system was generated. Partial deletion of the SARS-CoV-S protein cytoplasmic domain allowed efficient incorporation into VSV particles and led to the generation of a pseudotype (VSV-SARS-St19) at high titre. Green fluorescent protein expression was demonstrated as early as 7 h after infection of Vero E6 cells with VSV-SARS-St19. VSV-SARS-St19 was neutralized by anti-SARS-CoV antibody and soluble ACE2, and its infection was blocked by treatment of Vero E6 cells with anti-ACE2 antibody. These results indicated that VSV-SARS-St19 infection is mediated by SARS-CoV-S protein in an ACE2-dependent manner. VSV-SARS-St19 will be useful for analysing the function of SARS-CoV-S protein and for developing rapid methods of detecting neutralizing antibodies specific for SARS-CoV infection.

Publisher

Microbiology Society

Subject

Virology

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