Salipiger nanhaiensis sp. nov., a bacterium isolated from deep sea water

Abstract

A Gram-stain-negative, facultatively anaerobic, chemoheterotrophic, moderately halophilic, exopolysaccharide (EPS)-producing, cream, non-motile and rod-shaped bacterium, designated strain ZH114T, was isolated from deep water of the South China Sea, and was subjected to a polyphasic taxonomic study. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that this strain belongs to the genus Salipiger with the highest sequence similarity to Salipiger mucescens LMG 22090T (96.83 %), followed by Pseudodonghicola xiamenensis LMG 24574T (96.12 %). Growth occurred at 4–37 °C (optimum 32 °C), pH 6.0–10.0 (optimum pH 9.0–10.0) and in the presence of 0–19 % NaCl (w/v) (optimum 6 %, w/v). It did not produce poly-β-hydroxyalkanoate granules or bacteriochlorophyll a. Acid was produced from glycerol, erythrose, ribose, d-xylose, galactose, glucose, fructose, mannitol, cellobiose, maltose, lactose, melibiose, turanose, d-lyxose, d-tagatose, d-fucose, d-arabitol and l-arabitol after inoculating for 24 h and weakly positive results were also detected after 48 h in API 50CH strips with d-arabinose, l-arabinose, l-xylose, adonitol, mannose, aesculin, salicin, sucrose, mycose and l-fucose. The predominant fatty acids were C18 : 1ω7c and/or C18 : 1ω6c, C16 : 0, C18 : 0 and 11-methyl C18 : 1ω7c. The major polar lipids of ZH114T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified lipids. The major respiratory quinone was ubiquinone Q-10. The genomic DNA G+C content of strain ZH114T was 63.8 mol%. Based on this phenotypic, chemotaxonomic and phylogenetic analysis, strain ZH114T should be classified as a representative of a novel species of the genus Salipiger , for which the name Salipiger nanhaiensis sp. nov. is proposed. The type strain is ZH114T ( = JCM 19383T = KCTC 32468T).