Rufibacter roseus sp. nov., isolated from radiation-polluted soil

Abstract

A rose, Gram-stain-negative, aerobic, rod-shaped bacterium that was motile by gliding, and designated strain H359T, was isolated from radiation-polluted soil (with high Cs137) from the Xinjiang Uygur Autonomous Region of PR China and subjected to a polyphasic taxonomic analysis. The isolate grew optimally at 30 °C and pH 7.0. It grew with NaCl up to 4 % (w/v). 16S rRNA gene sequence analysis indicated that strain H359T belonged to the genus Rufibacter , a member of the family Cytophagaceae , with Rufibacter tibetensis CCTCC AB 208084T as its closest phylogenetic relative, having 96.1 % 16S rRNA gene sequence similarity to the type strain. Strain H359T contained menaquinone-7 (MK-7) as the predominant menaquinone, and the major fatty acids were iso-C15 : 0, summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 1ω5c. The polar lipid profile had phosphatidylethanolamine as the major component. The DNA G+C content was 43.9 mol%. Based on phenotypic, genotypic and phylogenetic evidence, strain H359T represents a novel species of the genus Rufibacter , for which the name Rufibacter roseus sp. nov. is proposed. The type strain is H359T ( = CPCC 100615T = KCTC 42217T).