Burkholderia susongensis sp. nov., a mineral-weathering bacterium isolated from weathered rock surface

Abstract

A novel type of mineral-weathering bacterium was isolated from the weathered surface of rock (mica schist) collected from Susong (Anhui, China). Cells of strain L226T were Gram-stain-negative. The strain grew optimally at 30 °C, with 1 % (w/v) NaCl and at pH 7.0 in trypticase soy broth. On the basis of 16S rRNA gene phylogeny, strain L226T was shown to belong to the genus Burkholderia and the closest phylogenetic relatives were Burkholderia sprentiae WSM5005T (98.3 %), Burkholderia acidipaludis NBRC 101816T (98.2 %), Burkholderia tuberum STM678T (97.2 %) and Burkholderia diazotrophica JPY461T (97.1 %). The DNA G+C content was 63.5 mol% and the respiratory quinone was Q-8. The major fatty acids were C16 : 0, C17 : 0 cyclo and C19 : 0 cyclo ω8c. The polar lipid profile of strain L226T consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, unknown lipids and unidentified aminophospholipids. Based on the low level of DNA–DNA relatedness (ranging from 25.8 % to 34.4 %) to the tested type strains of species of the genus Burkholderia and unique phenotypic characteristics, it is suggested that strain L226T represents a novel species of the genus Burkholderia , for which the name Burkholderia susongensis sp. nov., is proposed. The type strain is L226T ( = CCTCC AB2014142T = JCM 30231T).