Pedobacter daejeonensis sp. nov. and Pedobacter trunci sp. nov., isolated from an ancient tree trunk, and emended description of the genus Pedobacter

Abstract

Two Gram-stain-negative, yellow, aerobic and rod-shaped bacterial isolates, designated THG-DN3.18T and THG-DN3.19T, were isolated from an ancient tree trunk from Daejeon, South Korea. 16S rRNA gene sequence similarity showed that both strains belong to the genus Pedobacter within the family Sphingobacteriaceae . Strain THG-DN3.18T exhibited maximum sequence similarity with Pedobacter boryungensis KCTC 23344T (98.5 %) while strain THG-DN3.19T exhibited maximum sequence similarity with Pedobacter nyackensis LMG 24260T (97.3 %). In DNA–DNA hybridization tests, the two strains showed less than 35 % relatedness with respect to closely related species of the genus Pedobacter . Both strains contained iso-C15 : 0 and C16 : 1ω6c and/or C16 : 1ω7c (summed feature 3) as the predominant fatty acids and MK-7 as the major isoprenoid quinone. The DNA G+C contents of strains THG-DN3.18T and THG-DN3.19T were 35.5 and 40.1 mol%, respectively. The genotypic analysis, biochemical properties, and phenotypic and chemotaxonomic characteristics indicate that strains THG-DN3.18T and THG-DN3.19T represent novel species of the genus Pedobacter , for which the names Pedobacter daejeonensis sp. nov. and Pedobacter trunci sp. nov. are proposed. The type strains are THG-DN3.18T ( = KCTC 42230T = JCM 30352T) and THG-DN3.19T ( = KCTC 42233T = JCM 30353T), respectively.