Affiliation:
1. NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune-411008, Maharashtra, India
2. Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh-160036, India
3. Microbial Culture Collection (MCC), National Centre for Cell Science, Pune 411007, Maharashtra, India
Abstract
A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium designated SGD-V-25T was isolated from Veraval sediment sample, India. Strain SGD-V-25T was capable of growing at 25–50 °C (optimum 37 °C), pH 6–12 (optimum pH 7.0) and with 0–5 % (w/v) NaCl. The taxonomic position of this strain was deduced using a polyphasic approach and the 16S rRNA gene sequence analysis showed that the isolate belongs to the phylum
Firmicutes
, forming the cluster with
Bacillus badius
MTCC 1548T, with which it shares highest similarity of 99.1 % with 13 nt differences. Other type strains of the genus
Bacillus
showed less than 96 % similarity. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid. The polar lipid profile of strain SGD-V-25T showed the presence of diphosphatidylglycerol, phosphatidylglycerol, phsophoglycolipid and two aminophospholipids. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0, C16 : 1ω11c and C16 : 0. The genomic DNA G+C content of strain SGD-V-25T was 37.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA hybridization, strain SGD-V-25T could be clearly distinguished from closely related members of the genus
Bacillus
, and the name Bacillus encimensis sp. nov., is proposed to accommodate this strain. The type strain is SGD-V-25T ( = NCIM 5513T = DSM 28241T).
Funder
CSIR
Council for Scientific and Industrial Research
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
15 articles.
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