Niveispirillum cyanobacteriorum sp. nov., a nitrogen-fixing bacterium isolated from cyanobacterial aggregates in a eutrophic lake

Abstract

A Gram-stain-negative, slightly curved rod-shaped, non-spore-forming diazotrophic bacterium, designated strain TH16T, was isolated from cyanobacterial aggregates taken from eutrophic Lake Taihu, Jiangsu Province, China. The pH range for growth was 5–9 (optimum at pH 7.0), salinity range was 0–2  % (w/v) NaCl (optimum 0  %) and temperature range was 20–37 °C (optimum 30 °C) in nutrient broth. Phylogenetic analysis indicated that strain TH16T clusters near and is closely related to the genus Niveispirillum within the family Rhodospirillaceae of the class Alphaproteobacteria. Within the genus Niveispirillum, strain TH16T was related most closely to Niveispirillum irakense KBC1T (98.1  % 16S rRNA gene sequence similarity) and Niveispirillum fermenti CC-LY736T (97.0  %). The DNA G+C content of strain TH16T was 64 mol%. DNA–DNA relatedness between strain TH16T and the type strains of N. irakense and N. fermenti was 39.6 and 30.1  %, respectively. The major respiratory quinone was ubiquinone Q-10.The major fatty acids (>10  %) were C18  :  1ω6c/C18  :  1ω7c, C18  :  1 2-OH and C16  :  0 3-OH. Genes in the puf operon, encoding proteins of the photosynthetic reaction centre and core light-harvesting complexes, were also present. Based on morphological, chemotaxonomic and phylogenetic data, strain TH16T represents a novel species within the genus Niveispirillum, for which the name Niveispirillum cyanobacteriorum sp. nov. is proposed. The type strain is TH16T ( = CGMCC 1.12958T = LMG 28334T).