Sinomonas susongensis sp. nov., isolated from the surface of weathered biotite

Abstract

A novel actinomycete, designated strain A31T, was isolated from the surface of weathered biotite in Susong, Anhui Province, China. The organism grew optimally at 30 °C, at pH 8.0 and with 1 % (w/v) NaCl. Strain A31T had A3α as the cell-wall peptidoglycan type and galactose, mannose and rhamnose as whole-cell sugars. Anteiso-C15 : 0 and anteiso-C17 : 0 were the major cellular fatty acids and MK-9(H2) was the predominant respiratory quinone. In addition, the total polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylmonomethylethanolamine and four glycolipids. The genomic DNA G+C content of strain A31T was 70.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A31T was related most closely to Sinomonas albida LC13T (98.3 % similarity), Sinomonas atrocyanea DSM 20127T (98.2 %), Sinomonas soli CW 59T (98.1 %), Sinomonas flava CW 108T (97.8 %), ‘Sinomonas mesophila’ MPKL 26 (97.3 %), Sinomonas echigonensis LC10T (97.1 %) and ‘ Sinomonas notoginsengisoli ’ SYP-B575 (96.7 %). DNA–DNA hybridization studies with the new isolate showed relatedness values of 16.0–56.6 % with its six closest neighbours. Based on phenotypic, chemotaxonomic and phylogenetic analysis, strain A31T represents a novel species of the genus Sinomonas , for which the name Sinomonas susongensis sp. nov. is proposed. The type strain is A31T ( = DSM 28245T = CCTCC AB 2014068T).