The Borrelia burgdorferi outer-surface protein ErpX binds mammalian laminin

Abstract

The Lyme disease spirochaete,Borrelia burgdorferi, can invade and persistently infect its hosts' connective tissues. We now demonstrate thatB. burgdorferiadheres to the extracellular matrix component laminin. The surface-exposed outer-membrane protein ErpX was identified as having affinity for laminin, and is the first laminin-binding protein to be identified in a Lyme disease spirochaete. The adhesive domain of ErpX was shown to be contained within a small, unstructured hydrophilic segment at the protein's centre. The sequence of that domain is distinct from any previously identified bacterial laminin adhesin, suggesting a unique mode of laminin binding.