Protection and antibody responses in different strains of mouse immunized with plasmid DNAs encoding influenza virus haemagglutinin, neuraminidase and nucleoprotein-Reference-Cited by-同舟云学术

Protection and antibody responses in different strains of mouse immunized with plasmid DNAs encoding influenza virus haemagglutinin, neuraminidase and nucleoprotein

Published:1999-10-01 Issue:10 Volume:80 Page:2559-2564
ISSN:0022-1317
Container-title:Journal of General Virology
language:en
Short-container-title:

Author:

Chen Ze¹,Yoshikawa Tomoki¹,Kadowaki Shin-etsu¹,Hagiwara Yukari¹,Matsuo Kazutoshi¹,Asanuma Hideki¹,Aizawa Chikara²,Kurata Takeshi¹,Tamura Shin-ichi¹

Affiliation:

1. Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan 1

2. Research Center for Biologicals, Kitasato Institute, 6-111 Arai, Kitamoto-shi, Saitama 364-0026, Japan2

Abstract

Protection against influenza virus infection and antibody responses in mice vaccinated with plasmid DNAs encoding haemagglutinin (HA), neuraminidase (NA) and nucleoprotein (NP) were compared among BALB/c (H-2d), B10 (H-2b) and C3H (H-2k ) mice. Mice were inoculated with each DNA construct twice, 3 weeks apart, at a dose of 1 μg per mouse by particle-mediated DNA transfer (gene gun) to the epidermis. They were challenged with a lethal dose of the homologous virus 7 days after the second vaccination. NA-DNA provided significant protection in all strains of mouse, whereas HA-DNA afforded significant protection only in BALB/c mice. The serum antibody titres against NA or HA molecules in BALB/c, C3H and B10 mice were high, intermediate and low, respectively. NP-DNA failed to provide protection in any strain of mouse, and elicited low titres of anti-NP antibodies. These results suggest that NA-DNA can be used as a vaccine component to provide effective protection against influenza virus infection in various strains of mouse.

Publisher

Microbiology Society

Subject

Virology

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