Determination of the complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore strain-Reference-Cited by-同舟云学术

Determination of the complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore strain

Published:2001-03-01 Issue:3 Volume:82 Page:647-653
ISSN:0022-1317
Container-title:Journal of General Virology
language:en
Short-container-title:

Author:

Tan Corrina¹,Huang Bin¹,Chang Siow Foong²,Ngoh Gim Hue²,Munday Barry³,Chen Shih Chu⁴,Kwang Jimmy¹

Affiliation:

1. Institute of Molecular Agrobiology, The National University of Singapore, 1 Research Link, Singapore1176041

2. Agri-food and Veterinary Authority, Animal Health Service Branch, Central Veterinary Laboratory, 60 Sengkang East Way, Singapore5485962

3. University of Tasmania, School of Biomedical Science, Locked Bag 1-320, Launceston, Tasmania 7250, Australia3

4. National Pingtung University of Science and Technology, Pingtung 91207, Taiwan, Republic of China4

Abstract

The complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus (GGNNV), Singapore strain, were determined. 5′RACE and RNA ligation were used to obtain the complete nucleotide sequences of the 5′ and 3′ non-coding regions (NCRs). GGNNV RNA1 was determined to be 3103 nt long, containing an ORF of 982 aa, while GGNNV RNA2 was determined to be 1433 nt long, containing an ORF of 338 aa. Both GGNNV RNAs are longer than those of other published betanodavirus sequences and the additional nucleotides were located within the NCRs. Analysis of GGNNV RNA2 revealed that it is closely related to redspotted grouper nervous necrosis virus and that both grouper viruses share the same neutralization epitope. Predicted domains for six RNA-dependent RNA polymerase motifs and two putative ORFs (proteins B1 and B2) were confirmed by sequence analysis of GGNNV RNA1.

Publisher

Microbiology Society

Subject

Virology

Reference31 articles.

1. The phylogenetic relationship of nervous necrosis virus from halibut ( Hippoglossus hippoglossus );Aspehaug;Bulletin of the European Association of Fish Pathologists,1999

2. Nodavirus;Ball,1994

3. Reverse genetics of nodaviruses . Advances in;Ball;Virus Research,1999

4. Picornavirus RNA translation: roles for cellular protein;Belsham;Trends in Microbiology,2000

5. Encephalomyelitis among turbot associated with a piconavirus-like agent;Bloch;Diseases of Aquatic Organisms,1991

Cited by 106 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A peptide scanned from the capsid protein of NNV induces effective immune response and protects pearl gentian groupers from viral nervous necrosis;Aquaculture;2023-07

2. Humoral immune response in Asian seabass vaccinated with inactivated and recombinant viral nervous necrosis vaccine;Aquaculture;2023-05

3. Identification and characterization of immunoglobulin tau (IgT) in Asian Seabass (Lates calcarifer) and mucosal immune response to nervous necrosis virus;Frontiers in Immunology;2023-02-20

4. Detection of nervous necrosis virus RGNNV genotype in pearl gentian grouper (Epinephelus lanceolatus ♂ × E. fuscoguttatus ♀) fry imported to Thailand;Aquaculture;2022-12

5. Construction of Attenuated Strains for Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) via Reverse Genetic System;Viruses;2022-08-06