Affiliation:
1. Freie Universität Berlin, Medizinische Klinik III, Hindenburgdamm 30, 12200 Berlin, Germany1
Abstract
Many C- and D-type retroviruses are known to cause a broad spectrum of malignant diseases in animals. Certain genome regions of these animal retroviruses are highly conserved between different animal species. It should be possible to detect new members of the retrovirus family with consensus PCR primers derived from these conserved sequence motifs. The consensus PCR primers developed in this study are generic enough to detect nearly all known oncogenic mammalian and avian exogenous C- and D-type retroviruses but do not amplify human endogenous retroviral sequences. In contrast to previous investigations, the present study involved highly stringent PCR conditions and truly generic PCR primers. Forty-four samples from patients with various immunophenotyped malignant diseases (acute and chronic T-/B-cell lymphocytic leukaemias, acute myeloid leukaemias, T-/B-cell lymphomas, chronic myeloproliferative disorders) and three cell lines (Hodgkin’s lymphoma, Burkitt’s lymphoma) have thus far been investigated using these PCR primers. The fact that no retroviruses have been found argues against an involvement of known animal oncoretroviruses or related hitherto undetected human retroviruses in the aetiopathogenesis of these diseases. The retrovirus detection system developed here may be used to confirm suspected retroviral involvement in other (malignant or nonmalignant) human diseases as well as to identify new animal retroviruses.
Reference39 articles.
1. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs;Altschul;Nucleic Acids Research,1997
2. Nuclear factors that bind to the U3 region of two murine myeloid leukemia-inducing retroviruses;Barat;Cas-Br-E and Graffi. Virology,1998
3. Proposals for the immunological classification of acute leukemias;Bene;European Group for the Immunological Characterization of Leukemias (EGIL). Leukemia,1995
4. Proposed revised criteria for the classification of acute myeloid leukemia. A report of the French–American–British Cooperative Group;Bennett;Annals of Internal Medicine,1985
5. Isolation of a type D retrovirus from B-cell lymphomas of a patient with AIDS;Bohannon;Journal of Virology,1991
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