Evidence for RNA-mediated defence effects on the accumulation of Potato leafroll virus

Author:

Barker Hugh1,McGeachy Kara D.1,Ryabov Eugene V.1,Commandeur Uli2,Mayo Mike A.1,Taliansky Michael1

Affiliation:

1. Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK1

2. Aachen University of Technology (RWTH), Institute for Biology VII, Molecular Biotechnology, Worringerweg 1, D-52074 Aachen, Germany2

Abstract

In plants infected with Potato leafroll virus (PLRV), or other luteoviruses, infection is very largely confined to cells in the vascular system. Even in tobacco plants transformed with PLRV full-length cDNA, in which all mesophyll cells should synthesize infectious PLRV RNA transcripts, only a minority of the mesophyll cells accumulate detectable amounts of virus. We have explored this phenomenon further by transforming a better PLRV host, Nicotiana benthamiana, with the same transgene, by superinfecting transformed plants with Potato virus Y and by producing tobacco plants in which cells contained both PLRV cDNA and DNA encoding the P1/HC-Pro genes of the potyvirus Tobacco etch virus. A greater proportion of cells in superinfected plants or in doubly transgenic plants accumulated PLRV than did in singly transgenic tobacco plants. However, most cells in these plants did not accumulate virus. To investigate restriction of the multiplication of viruses containing PLRV sequences, transgenic plants were infected with a chimeric virus that consisted of Tobacco mosaic virus (TMV) containing genes for either the coat protein (CP) of PLRV or jellyfish green fluorescent protein (GFP) in place of the TMV coat protein. The virus that encoded PLRV CP spread more slowly and accumulated less extensively than did the virus that expressed GFP. The results support the suggestion that an RNA-mediated form of resistance that resembles post-transcriptional gene silencing operates in non-vascular cells and may be part of the mechanism that restricts PLRV to vascular tissue in conventionally infected plants.

Publisher

Microbiology Society

Subject

Virology

Reference32 articles.

1. A viral suppressor of gene silencing in plants;Anandalakshmi;Proceedings of the National Academy of Sciences, USA,1998

2. Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA;Angell;EMBO Journal,1997

3. Systemic infection by a phloem-restricted virus in parenchyma cells in mixed infection;Atabekov;Biologicheskie nauki,1984

4. Invasion of non-phloem tissue in Nicotiana clevelandii by potato leafroll luteovirus is enhanced in plants also infected with potato Y potyvirus;Barker;Journal of General Virology,1987

5. Specificity of the effect of sap-transmissible viruses in increasing the accumulation of luteoviruses in co-infected plants;Barker;Annals of Applied Biology,1989

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3