Affiliation:
1. Laboratory of Molecular Genetics of Microorganisms, Oswaldo Cruz Institute, Rio de Janeiro, Brazil
Abstract
The mobilome plays a crucial role in bacterial adaptation and is therefore a starting point to understand and establish the gene flow occurring in the process of bacterial evolution. This is even more so if we consider that the mobilome of environmental bacteria can be the reservoir of genes that may later appear in the clinic. Recently, new genera have been proposed in the family
Mycobacteriaceae
, including the genus
Mycolicibacterium
, which encompasses dozens of species of agricultural, biotechnological, clinical and ecological importance, being ubiquitous in several environments. The current scenario in the
Mycobacteriaceae
mobilome has some bias because most of the characterized mycobacteriophages were isolated using a single host strain, and the few plasmids reported mainly relate to the genus
Mycobacterium
. To fill in the gaps in these issues, we performed a systematic in silico study of these mobile elements based on 242 available genomes of the genus
Mycolicibacterium
. The analyses identified 156 putative plasmids (19 conjugative, 45 mobilizable and 92 non-mobilizable) and 566 prophages in 86 and 229 genomes, respectively. Moreover, a contig was characterized by resembling an actinomycete integrative and conjugative element (AICE). Within this diversity of mobile genetic elements, there is a pool of genes associated with several canonical functions, in addition to adaptive traits, such as virulence and resistance to antibiotics and metals (mercury and arsenic). The type-VII secretion system was a common feature in the predicted plasmids, being associated with genes encoding virulent proteins (EsxA, EsxB, PE and PPE). In addition to the characterization of plasmids and prophages of the family
Mycobacteriaceae
, this study showed an abundance of these genetic elements in a dozen species of the genus
Mycolicibacterium
.
Funder
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Fundação Oswaldo Cruz
Reference115 articles.
1. Phylogenomics and comparative genomic studies robustly support division of the genus mycobacterium into an emended genus mycobacterium and four novel genera [published correction appears in Front Microbiol. 2019 Apr 09;10:714];Gupta;Front Microbiol,2018
2. Genomics of Atlantic forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS;Morgado;Microb Genom,2020
3. Plasmid-Mediated horizontal gene transfer is a coevolutionary process;Harrison;Trends Microbiol,2012
4. Crosstalk between vertical and horizontal gene transfer: plasmid replication control by a conjugative relaxase;Lorenzo-Díaz;Nucleic Acids Res,2017
5. Native plasmid-encoded mercury resistance genes are functional and demonstrate natural transformation in environmental bacterial isolates;Kothari;mSystems,2019
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