Affiliation:
1. Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin 300384, PR China
2. Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Terengganu 21030, Malaysia
3. Tianjin Hengqian Aquaculture Co. Ltd, Tianjin 300270, PR China
Abstract
The virulence of
Vibrio parahaemolyticus
is variable depending on its virulence determinants. A
V. parahaemolyticus
strain, in which the virulence is governed by the pirA and pirB genes, can cause acute hepatopancreatic necrosis disease (AHPND) in shrimps. Some
V. parahaemolyticus
that are non-AHPND strains also cause shrimp diseases and result in huge economic losses, while their pathogenicity and pathogenesis remain unclear. In this study, a non-AHPND
V. parahaemolyticus
, TJA114, was isolated from diseased Penaeus vannamei associated with a high mortality. To understand its virulence and adaptation to the external environment, whole-genome sequencing of this isolate was conducted, and its phenotypic profiles including pathogenicity, growth characteristics and nutritional requirements were investigated. Shrimps following artificial infection with this isolate presented similar clinical symptoms to the naturally diseased ones and generated obvious pathological lesions. The growth characteristics indicated that the isolate TJA114 could grow well under different salinity (10–55 p.p.t.), temperature (23–37 °C) and pH (6–10) conditions. Phenotype MicroArray results showed that this isolate could utilize a variety of carbon sources, amino acids and a range of substrates to help itself adapt to the high hyperosmotic and alkaline environments. Antimicrobial-susceptibility test showed that it was a multidrug-resistant bacterium. The whole-genomic analysis showed that this
V. parahaemolyticus
possessed many important functional genes associated with multidrug resistance, stress response, adhesions, haemolysis, putative secreted proteases, dedicated protein secretion systems and a variety of nutritional metabolic mechanisms. These annotated functional genes were confirmed by the phenotypic profiles. The results in this study indicated that this
V. parahaemolyticus
isolate possesses a high pathogenicity and strong environmental adaptability.
Funder
the Innovation Team of Tianjin Fisheries Research System
The Scientific Programs of Tianjin City
the Science and Technology Innovation Project of Shandong province
the Science and Technology Project in the Field of Social Development in Tianjin Binhai New Area
the Scientific Research and Innovation Project of Tianjin Agricultural University in 2019
Cited by
9 articles.
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