Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children-Reference-Cited by-同舟云学术

Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children

Published:2007-09-01 Issue:9 Volume:56 Page:1185-1188
ISSN:0022-2615
Container-title:Journal of Medical Microbiology
language:en
Short-container-title:

Author:

Dias Cícero A.¹²,Teixeira Lúcia Martins¹,Carvalho Maria da Glória³,Beall Bernard³

Affiliation:

1. Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil

2. Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, and Hospital Mãe de Deus, Porto Alegre, Rio Grande do Sul, Brazil

3. Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA

Abstract

Capsular serotype surveillance of clinical isolates of Streptococcus pneumoniae is essential for evaluation of the potential impact of introducing multivalent capsular serotype-based vaccines in Latin America. Here, a previously described sequential multiplex PCR method was revised for optimal targeting of prevalent serotypes in Latin America. The revised protocol successfully serotyped 139/147 pneumococci (94.6 %) from Brazilian children, demonstrating a labour-efficient, accurate method requiring only conventional PCR capability.

Publisher

Microbiology Society

Subject

Microbiology (medical),General Medicine,Microbiology

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