Intraspecific mitochondrial DNA polymorphism within the emerging filamentous fungal pathogen Trichoderma longibrachiatum

Author:

Antal Zsuzsanna1,Varga János,Kredics László1,Szekeres András,Hatvani Lóránt,Manczinger László,Vágvölgyi Csaba,Nagy Elisabeth1

Affiliation:

1. Microbiological Research Group, Hungarian Academy of Sciences and University of Szeged, PO Box 533, H-6701 Szeged, Hungary

Abstract

The genetic diversity of the emerging fungal pathogen Trichoderma longibrachiatum was examined at the level of mitochondrial DNA. The 17 investigated strains, comprising nine clinical and eight non-clinical isolates, exhibited seven and ten different mitochondrial DNA profiles by using the restriction enzymes BsuRI and Hin6I, respectively. The sizes of mitochondrial DNAs varied from 34·9 to 39·5 kb. The discriminatory power of the method was higher than that of internal transcribed spacer sequence analysis and therefore should be more suitable for identification and epidemiological investigations. However, clinical and non-clinical isolates did not form separate clusters on the resulting dendrogram and thus there was no indication of a correlation between genetic structure and pathogenicity of the isolates.

Publisher

Microbiology Society

Subject

Microbiology (medical),General Medicine,Microbiology

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