A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture-Reference-Cited by-同舟云学术

A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture

Published:2006-06-01 Issue:6 Volume:152 Page:1601-1607
ISSN:1350-0872
Container-title:Microbiology
language:en
Short-container-title:

Author:

O'Connell Catherine M.¹,Nicks Kristy M.¹

Affiliation:

1. Department of Microbiology and Immunology, UAMS, 4301 West Markham, Little Rock, AR 72205, USA

Abstract

A highly conserved cryptic plasmid is present inChlamydia trachomatisyet naturally occurring plasmid-deficient isolates are very rare. This paper describes the isolation and characterization of a plasmid-deficient strain ofC. muridarum, using novobiocin as a curing agent. Plasmid-deficient derivatives ofC. muridarumstrain Nigg were generated at high efficiencies (4–30 %). Phenotypic characterization revealed that the cured derivative was unable to accumulate glycogen within intracytoplasmic inclusions. In addition, this strain formed small plaques at a reduced efficiency compared to the wild-type parent.

Publisher

Microbiology Society

Subject

Microbiology

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