Belo Horizonte virus: a vaccinia-like virus lacking the A-type inclusion body gene isolated from infected mice-Reference-Cited by-同舟云学术

Belo Horizonte virus: a vaccinia-like virus lacking the A-type inclusion body gene isolated from infected mice

Published:2004-07-01 Issue:7 Volume:85 Page:2015-2021
ISSN:0022-1317
Container-title:Journal of General Virology
language:en
Short-container-title:

Author:

Trindade Giliane S.¹,da Fonseca Flávio G.²,Marques João T.³,Diniz Sueli⁴,Leite Juliana A.¹,De Bodt Stefanie⁵,Van der Peer Yves⁵,Bonjardim Cláudio A.¹,Ferreira Paulo C. P.¹,Kroon Erna G.¹

Affiliation:

1. Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Caixa postal 486, CEP 31270-901, Belo Horizonte, MG, Brazil

2. Laboratório de Imunologia Celular e Molecular, Centro de Pesquisas René Rachou – FIOCRUZ, Avenida Augusto de Lima 1715, CEP 30190-002, Belo Horizonte, MG, Brazil

3. Department of Cancer Biology, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA

4. Laboratório de Biologia de Microrganismos, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Caixa postal 486, CEP 31270-901, Belo Horizonte, MG, Brazil

5. Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Technologiepark 927, B-9052 Ghent, Belgium

Abstract

Here is described the isolation of a naturally occurring A-type inclusion body (ATI)-negative vaccinia-like virus, Belo Horizonte virus (VBH), obtained from a mousepox-like outbreak in Brazil. The isolated virus was identified and characterized as an orthopoxvirus by conventional methods. Molecular characterization of the virus was done by DNA cross-hybridization using Vaccinia virus (VACV) DNA. In addition, conserved orthopoxvirus genes such as vaccinia growth factor, thymidine kinase and haemagglutinin were amplified by PCR and sequenced. All sequences presented high similarity to VACV genes. Based on the sequences, phenograms were constructed for comparison with other poxviruses; VBH clustered consistently with VACV strains. Attempts to amplify the ATI gene (ati) by PCR, currently used to identify orthopoxviruses, were unsuccessful. Results presented here suggest that most of the ati gene is deleted in the VBH genome.

Publisher

Microbiology Society

Subject

Virology

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