Affiliation:
1. Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany
2. Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V., Hans-Knöll-Institut., D-07745 Jena, Germany
3. Department of Entomology and Plant Pathology, Auburn University, Auburn, AL 36849, USA
Abstract
A yellow, Gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain JM-1T) was isolated from the rhizosphere of a field-grown Zea mays plant in Auburn, AL, USA. 16S rRNA gene sequence analysis of strain JM-1T showed high sequence similarity to the type strains of
Novosphingobium capsulatum
(98.9 %),
Novosphingobium aromaticivorans
(97.4 %),
Novosphingobium subterraneum
(97.3 %) and
Novosphingobium taihuense
(97.1 %); sequence similarities to all other type strains of species of the genus
Novosphingobium
were below 97.0 %. DNA–DNA hybridizations of strain JM-1T and
N. capsulatum
DSM 30196T,
N. aromaticivorans
SMCC F199T and
N. subterraneum
SMCC B0478T showed low similarity values of 33 % (reciprocal: 21 %), 14 % (reciprocal 16 %) and 36 % (reciprocal 38 %), respectively. Ubiquinone Q-10 was detected as the major respiratory quinone. The predominant fatty acid was C18 : 1ω7c (71.0 %) and the typical 2-hydroxy fatty acid C14 : 0 2-OH (11.7 %) was detected. The polar lipid profile contained the diagnostic lipids diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and phosphatidylcholine. Characterization by 16S rRNA gene sequence analysis, physiological parameters, pigment analysis, and ubiquinone, polar lipid and fatty acid composition revealed that strain JM-1T represents a novel species of the genus
Novosphingobium
. For this species we propose the name Novosphingobium rhizosphaerae sp. nov. with the type strain JM-1T ( = LMG 28479T = CCM 8547T).
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
23 articles.
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