A simple PCR assay for the identification of the novel Streptococcus pneumoniae serotype 7D-Reference-Cited by-同舟云学术

A simple PCR assay for the identification of the novel Streptococcus pneumoniae serotype 7D

Published:2023-08-08 Issue:8 Volume:72 Page:
ISSN:0022-2615
Container-title:Journal of Medical Microbiology
language:en
Short-container-title:

Author:

Maruhn Karsten¹²^ORCID,Itzek Andreas²,Imoehl Matthias¹,van der Linden Mark²

Affiliation:

1. Laboratory Diagnostic Center, University Hospital RWTH Aachen, Aachen, Germany

2. National Reference Center for Streptococci, Institute of Medical Microbiology, University Hospital RWTH, Aachen, Germany

Abstract

The identification of the novel pneumococcal serotype 7D by Neufeld quellung reaction requires significant expertise. To circumvent this, we developed a simple serotype-specific PCR method to discriminate serotype 7D from the closely related serotypes 7C, 7B and 40. The established PCR was validated with the strain collection of the German National Reference Center for Streptococci (GNRCS). However, no isolate initially assigned as serotype 7B, 7C or 40 was identified as serotype 7D.

Publisher

Microbiology Society

Subject

Microbiology (medical),General Medicine,Microbiology

Link

https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001739?crawler=true&mimetype=application/pdf

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