Synoviocytes assist in modulating the effect of Ross River virus infection in micromass-cultured primary human chondrocytes

Abstract

Graphical abstract A schematic representation of the study methodology. Human micromass-cultured chondrocytes were either directly infected with RRV or secondarily through co-culture with RRV-infected FLS. Micromass chondrocytes were analysed after 1, 3 and 5 days post-infection for gene expression using a PCR array and for viral load using genome copy number qPCR. FLS were analysed for viral load via qPCR, and supernatants were analysed for infectious viral particles using a plaque assay.