Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons-Reference-Cited by-同舟云学术

Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons

Published:2014-11-01 Issue:11 Volume:95 Page:2462-2467
ISSN:0022-1317
Container-title:Journal of General Virology
language:en
Short-container-title:

Author:

Aubry Fabien¹,Nougairède Antoine¹,de Fabritus Lauriane¹,Querat Gilles¹,Gould Ernest A.¹,de Lamballerie Xavier¹

Affiliation:

1. Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 ‘Emergence des Pathologies Virales’, 13385 Marseille, France

Abstract

Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified infectious RNA viruses were generated in days using the newly described ISA (infectious-subgenomic-amplicons) method. This new versatile and simple procedure may enhance our capacity to obtain infectious RNA viruses from PCR-amplified genetic material.

Publisher

Microbiology Society

Subject

Virology

Reference22 articles.

1. Complete Genome of a Genotype I Japanese Encephalitis Virus Isolated from a Patient with Encephalitis in Vientiane, Lao PDR

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