Lymantria dispar iflavirus 1 (LdIV1), a new model to study iflaviral persistence in lepidopterans

Author:

Carrillo-Tripp Jimena1,Krueger Elizabeth N.1,Harrison Robert L.2,Toth Amy L.3,Miller W. Allen1,Bonning Bryony C.3

Affiliation:

1. Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011, USA

2. Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service, Beltsville, MD 20705, USA

3. Department of Entomology, Iowa State University, Ames, IA 50011, USA

Abstract

The cell line IPLB-LD-652Y, derived from the gypsy moth (Lymantria dispar L.), is routinely used to study interactions between viruses and insect hosts. Here we report the full genome sequence and biological characteristics of a small RNA virus, designated Lymantria dispar iflavirus 1 (LdIV1), that was discovered to persistently infect IPLB-LD-652Y. LdIV1 belongs to the genus Iflavirus. LdIV1 formed icosahedral particles of approx. 30 nm in diameter and contained a 10 044 nt polyadenylated, positive-sense RNA genome encoding a predicted polyprotein of 2980 aa. LdIV1 was induced by a viral suppressor of RNA silencing, suggesting that acute infection is restricted by RNA interference (RNAi). We detected LdIV1 in all tested tissues of gypsy-moth larvae and adults, but the virus was absent from other L. dispar-derived cell lines. We confirmed LdIV1 infectivity in two of these cell lines (IPLB-LD-652 and IPLB-LdFB). Our results provide a novel system to explore persistent infections in lepidopterans and a new model for the study of iflaviruses, a rapidly expanding group of viruses, many of which covertly infect their hosts.

Publisher

Microbiology Society

Subject

Virology

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