Parasutterella secunda sp. nov., isolated from human faeces and proposal of Sutterellaceae fam. nov. in the order Burkholderiales

Abstract

A novel, strictly anaerobic, non-spore-forming, Gram-reaction-negative coccobacillus bacterium, designated strain YIT 12071T, was isolated from human faeces. Biochemically, this strain was largely unreactive and asaccharolytic. Growth of this strain in peptone-yeast-extract broth was weak, producing no visible turbidity, and no short-chain fatty acids were detected as an end product of metabolism. Following 16S rRNA gene sequence analysis, strain YIT 12071Twas found to be most closely related toParasutterella excrementihominis(90 % sequence similarity) and phylogenetically distinct from other known genera belonging to the orderBurkholderiales. Biochemical data supported the affiliation of this strain with the genusParasutterella. Strain YIT 12071T, therefore, represents a novel species of the genusParasutterella, for which the nameParasutterella secundasp. nov. is proposed. The type strain is YIT 12071T(=DSM 22575T=JCM 16078T). On the basis of 16S rRNA gene sequence analysis, species of the generaSutterellaandParasutterellaform a distinct and deep evolutionary lineage of descent in the orderBurkholderiales. This lineage could not be associated with any of the four known families of the orderBurkholderiales. The distinct phylogenetic position and the unusual combination of chemotaxonomic characteristics shared by these genera, such as the predominant quinones and cellular fatty acid compositions, suggest that they constitute a novel family in the orderBurkholderiales, for which the nameSutterellaceaefam. nov. is proposed to accommodate the generaSutterellaandParasutterella.