Actinoallomurus oryzae sp. nov., an endophytic actinomycete isolated from roots of a Thai jasmine rice plant

Abstract

The novel strain GMKU 370T was isolated from roots of a Thai jasmine rice plant (Oryza sativa L. ‘KDML 105’) collected from Pathum Thani Rice Research Center, Pathum Thani province, Thailand. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GMKU 370T formed a distinct clade within the genus Actinoallomurus. Strain GMKU 370T contained meso-diaminopimelic acid and lysine in the cell-wall peptidoglycan and galactose and madurose as whole-cell sugars. No mycolic acids were detected. The predominant menaquinones were MK-9(H6) and MK-9(H8). The polar phospholipids consisted of phosphatidylglycerol and phosphatidylinositol. These chemical properties reveal that strain GMKU 370T belongs to the genus Actinoallomurus. Strain GMKU 370T is distinct from the phylogenetically closely related type strains Actinoallomurus iriomotensis NBRC 103685T and Actinoallomurus coprocola NBRC 103688T (99.20 % 16S rRNA gene sequence similarity to both) in terms of differences in phenotypic characteristics and DNA–DNA relatedness (51 and 17 %, respectively). It is proposed that strain GMKU 370T represents a novel species of the genus Actinoallomurus, for which the name Actinoallomurus oryzae sp. nov. is proposed. The type strain is GMKU 370T ( = BCC 31373T  = NBRC 105246T).