Affiliation:
1. Division of Applied Life Science (BK 21), Plant Molecular Biology and Biotechnology Research Center, Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea
2. JGreen Inc., Department of Research & Development, Changnyeong 635-806, Republic of Korea
3. Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea
Abstract
A Gram-negative, non-spore-forming, endophytic bacterium, strain YC6886T, was isolated from the root of a halophyte,Rosa rugosa, which inhabits coastal areas of Namhae Island off the southern coast of Korea. Cells were non-motile, obligately aerobic rods and formed pale-yellow colonies. The isolate grew at 4–32 °C (optimum 25–28 °C) and at pH 6.5–9.5 (optimum pH 7.5) and grew optimally with 2–3 % (w/v) NaCl, but NaCl was not an absolute requirement for growth. Strain YC6886Tproduced yellow carotenoid pigments. Strain YC6886Texhibited the highest 16S rRNA gene sequence similarity withHaloferula sargassicolaMN1-1037T(97.4 %). Sequence similarities between strain YC6886Tand other members of the genusHaloferulawere 93.9–94.7 %. DNA–DNA relatedness between strain YC6886TandH. sargassicolaKCTC 22202TandHaloferula roseaKCTC 22201Twas 27 and 15 %, respectively. The major fatty acids were iso-C14 : 0, C16 : 0and C16 : 1ω9cand minor components were C14 : 0, C18 : 0and anteiso-C15 : 0. The major respiratory quinone was menaquinone 9 and the DNA G+C content was 58.5 mol%. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid and an unknown phosphoglycolipid. On the basis of phenotypic, chemotaxonomic, DNA–DNA hybridization and phylogenetic analysis, strain YC6886Trepresents a novel species in the genusHaloferula, for which the nameHaloferula luteolasp. nov. is proposed. The type strain is YC6886T( = KCTC 22447T = DSM 21608T). An emended description of the genusHaloferulais also presented.
Funder
Ministry of Knowledge Economy, Korea
Ministry of Education, Science and Technology, Korea
Brain Korea 21 project
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
16 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献