Laceyella sediminis sp. nov., a thermophilic bacterium isolated from a hot spring

Abstract

A thermophilic bacterium, designated strain RHA1T, was isolated from a sediment sample collected from a hot spring in Tengchong county, Yunnan province, south-west China, and was characterized by using a polyphasic approach. Based on its phenotypic and phylogenetic characteristics, strain RHA1T was affiliated to the genus Laceyella. The strain formed white aerial and yellow–white substrate mycelia, bearing single endospores on short sporophores. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Whole-cell hydrolysates contained ribose and glucose. The major fatty acids were iso-C15 : 0 (62.39 %) and anteiso-C15 : 0 (17.55 %). The predominant menaquinone was MK-9. The G+C content of the genomic DNA of strain RHA1T was 47.9 mol%. Based on DNA–DNA hybridization data, chemotaxonomic characteristics and differential physiological properties, strain RHA1T is considered to represent a novel species of the genus Laceyella, for which the name Laceyella sediminis sp. nov. is proposed; the type strain is RHA1T ( = DSM 45263T = CCTCC AA 208058T).