Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov.

Author:

Vermis Karen1,Coenye Tom2,LiPuma John J.3,Mahenthiralingam Eshwar4,Nelis Hans J.1,Vandamme Peter2

Affiliation:

1. Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium

2. Laboratory for Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium

3. Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109-0646, USA

4. Cardiff School of Biosciences, PO Box 915, Cardiff University, Cardiff CF10 3TL, UK

Abstract

Phenotypic and genotypic studies revealed new tools for differentiating Burkholderia cepacia genomovar VI from Burkholderia multivorans and other B. cepacia-complex species. Hence, the name Burkholderia dolosa sp. nov. is proposed, with LMG 18943T (=CCUG 47727T) as the type strain. B. dolosa can be differentiated from other B. cepacia-complex bacteria by its inability to assimilate tryptamine, azelaic acid and salicin and by its failure to grow on the B. cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification.

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

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