Myceligenerans halotolerans sp. nov., an actinomycete isolated from a salt lake, and emended description of the genus Myceligenerans

Abstract

A halotolerant actinomycete strain, designated XJEEM 11063T, was isolated from a salt lake in Xinjiang province, north-western China. Strain XJEEM 11063T grew at pH 6.0–8.0 (optimal growth at pH 7.0), between 10 and 40 °C (optimal growth at 28–37 °C) and at salinities of 0–10 % (w/v) NaCl (optimal growth at 0–5 %, w/v). The peptidoglycan type was A4α, and the whole-cell hydrolysates contained glucose, mannose and arabinose. The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. MK-9(H4) was the predominant menaquinone and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids and three unknown glycolipids. The genomic DNA G+C content was 71.8 mol%. The chemotaxonomic properties supported the affiliation of strain XJEEM 11063T to the genus Myceligenerans. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to Myceligenerans xiligouense XLG9A10.2T (98.3 %) and Myceligenerans crystallogenes DSM 17134T (97.0 %). However, it had relatively low values for DNA–DNA relatedness with the above strains (56.0 % and 47.5 %, respectively). Thus, on the basis of the results from this study, a novel species, Myceligenerans halotolerans sp. nov., is proposed. The type strain is XJEEM 11063T ( = DSM 21949T = CCTCC AA 208063T).