Yaniella fodinae sp. nov., isolated from a coal mine

Abstract

An orange bacterial strain, designated G5T, was isolated during the study of the bacterial diversity of a coal mine. The cell wall of strain G5T contained peptidoglycan type A4α (l-Lys–Gly–l-Glu) and the sugars xylose and mannose. The major menaquinones were MK-8 (45.0 %) and MK-9 (34.0 %) and minor amounts of MK-7 and MK-8(H2) were also found. The major fatty acids were anteiso-C15 : 0 (44.9 %) and iso-C15 : 0 (44.2 %). The main cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. 16S rRNA gene sequence analysis showed that strain G5T was closely related to Yaniella halotolerans YIM 70085T and Yaniella flava YIM 70178T (both 96.7 % 16S rRNA gene sequence similarity). The genomic DNA G+C content of strain G5T was 61.6 mol%. These data and other phenotypic characteristics clearly indicated that strain G5T represents a novel species of the genus Yaniella, for which the name Yaniella fodinae sp. nov. is proposed. The type strain is G5T (=MTCC 9846T=DSM 22966T).