Streptomyces youssoufiensis sp. nov., isolated from a Moroccan phosphate mine

Abstract

A novel actinomycete, strain X4T, was isolated from a phosphate mine in Youssoufia, 100 km north of Marrakesh, Morocco. The taxonomic status of this strain was evaluated by a polyphasic approach. Strain X4Thad white aerial mycelium withRectiflexibilesspore chains bearing smooth-surfaced spores and did not produce diffusible pigments. Chemotaxonomic analysis showed that the cell wall of strain X4Tcontainedll-diaminopimelic acid and glycine. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence indicated that strain X4Tbelongs to the Group I streptomycetes, branching off next toStreptomyces ramulosusNRRL B-2714TandStreptomyces kasugaensisM338-M1T. DNA–DNA relatedness and phenotypic data enabled strain X4Tto be distinguished from the phylogenetically most closely related type strains. It is therefore proposed that strain X4Trepresents a novel species of the genusStreptomyces, for which the nameStreptomyces youssoufiensissp. nov. is proposed; the type strain is X4T( = CCMM B709T = DSM 41920T).