Pacificibacter maritimus gen. nov., sp. nov., isolated from shallow marine sediment

Abstract

An aerobic, Gram-stain-negative, non-pigmented, non-motile bacterium, strain KMM 9031T, was isolated from a sandy sediment sample collected from the shore of the Sea of Japan and subjected to phenotypic and phylogenetic analysis. Based on comparative 16S rRNA gene sequence analysis, strain KMM 9031Tconstituted a separate phylogenetic line within theRoseobacterclade of the classAlphaproteobacteria, sharing highest sequence similarities with members of the generaRoseovarius(92.7–95.3 %),Pseudoruegeria(94.5 %),Sulfitobacter(92.7–94.4 %) andThalassobacter(94.2–94.3 %). The predominant fatty acid of strain KMM 9031Twas C18 : 1ω7c, with C16 : 0, C10 : 03-OH and C12 : 13-OH present in lesser amounts. The DNA G+C content of the isolate was 52.6 mol%. The major isoprenoid quinone was Q-10 and polar lipids comprised phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol and two unknown lipids. On the basis of phylogenetic analysis and physiological and biochemical characterization, strain KMM 9031Trepresents a novel species in a new genus, for which the namePacificibacter maritimusgen. nov., sp. nov. is proposed; the type strain is KMM 9031T( = NRIC 0785T = JCM 17096T).