Spongiibacterium flavum gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from the marine sponge Halichondria oshoro, and emended descriptions of the genera Croceitalea and Flagellimonas

Abstract

A Gram-negative, strictly aerobic, non-flagellated, non-gliding, oxidase- and catalase-positive, yellow-pigmented rod, designated A11T, was isolated from a marine sponge,Halichondria oshoro, collected on the coastline of Jeju Island, Republic of Korea. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that strain A11Twas a member of the familyFlavobacteriaceae. Its closest relatives were members of the generaMuricauda,FlagellimonasandCroceitalea(94.4–94.8 % 16S rRNA gene sequence similarity). The only polar lipid detected in strain A11Twas phosphatidylethanolamine. The dominant fatty acids were iso-C15 : 0(30.4 %), iso-C15 : 1G (26.7 %), iso-C17 : 03-OH (12.4 %) and iso-C15 : 03-OH (7.3 %). The DNA G+C content of strain A11Twas 41.7 mol% and its major respiratory quinone was MK-6. On the basis of combined data from phenotypic and phylogenetic analyses, strain A11Trepresents a novel genus and species within the familyFlavobacteriaceae, for which the nameSpongiibacterium flavumgen. nov., sp. nov. is proposed. The type strain of the type species is A11T( = KCTC 22665T = DSM 22638T). Emended descriptions of the generaCroceitaleaandFlagellimonasare also given.