Kangiella geojedonensis sp. nov., isolated from seawater

Abstract

A Gram-stain-negative, non-motile, non-spore-forming bacterial strain, YCS-5T, was isolated from seawater off the southern coast of Korea. Strain YCS-5T grew optimally at 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain YCS-5T fell within the clade comprising Kangiella species. Strain YCS-5T exhibited 16S rRNA gene sequence similarity values of 96.6, 95.7 and 97.9 % to the type strains of Kangiella koreensis, Kangiella aquimarina and Kangiella japonica, respectively, and less than 89.8 % to strains of other species used in the phylogenetic analysis. Strain YCS-5T contained Q-8 as the predominant ubiquinone and iso-C17 : 0, iso-C15 : 0, iso-C11 : 0 3-OH and iso-C17 : 1ω9c as the major fatty acids. The polar lipid profile of strain YCS-5T was similar to that of K. koreensis SW-125T, with phosphatidylglycerol and an unidentified aminolipid as major polar lipids. The DNA G+C content was 47 mol%. The mean DNA–DNA relatedness value between strain YCS-5T and K. japonica JCM 16211T was 12 %. Differential phenotypic properties and the phylogenetic and genetic distinctiveness of strain YCS-5T demonstrated that this strain is distinguishable from other Kangiella species. On the basis of the data presented, strain YCS-5T is considered to represent a novel species of the genus Kangiella, for which the name Kangiella geojedonensis sp. nov. is proposed; the type strain is YCS-5T ( = KCTC 23420T = CCUG 60526T).