Vibrio marisflavi sp. nov., isolated from seawater

Abstract

A Gram-reaction-negative, facultatively anaerobic bacterial strain, designated WH134T, was isolated from a seawater sample collected at a depth of 10 m near the Yellow Sea Cold Water Mass (YSCWM), 3 ° 59.970′ N 12 ° 0.157′ E, PR China. Cells of strain WH134T were slightly curved rods, motile by means of a polar flagellum and positive for poly-β-hydroxybutyrate (PHB) accumulation. The strain was able to grow in 1–6 % (w/v) NaCl, at pH 5–10 and 16–37 °C but not at 4 or 40 °C. The major cellular fatty acids were summed feature 3 C16  : 1 ω7c and/or iso-C15 : 0 2-OH, C16 : 0, C18 : 1 ω7c, C18 : 0 and C14 : 0. The DNA G+C content was 42.5 mol%. Phylogenetic analyses based on 16S rRNA, gyrB, topA, recA, ftsZ, mreB, gapA and rpoA gene sequences revealed that strain WH134T belongs to the genus Vibrio and showed gene sequence similarities of 96.6, 75.7, 74.6, 83.6, 78.9, 82.9, 86.0 and 89.4 % , respectively, to Vibrio rumoiensis S-1T. The possession of a flagellum, activity of arginine dihydrolase and lysine decarboxylase and inability to utilize citrate, however, differentiated strain WH134T from V. rumoiensis DSM 19141T. On the basis of the phenotypic, chemotaxonomic and phylogenetic evidence, strain WH134T represents a novel species of the genus Vibrio, for which the name Vibrio marisflavi sp. nov. is proposed. The type strain is WH134T (=CGMCC 1.8994T =LMG 25284T =DSM 23086T).