Chitinophaga eiseniae sp. nov., isolated from vermicompost

Abstract

A Gram-negative, rod-shaped bacterial strain, YC6729T, was isolated from vermicompost collected at Masan, Korea, and its taxonomic position was investigated by a polyphasic taxonomic approach. Strain YC6729Tgrew optimally at 30 °C and at pH 6.5–8.5. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YC6729Tbelongs to the genusChitinophagain the familyChitinophagaceae. It was related most closely toChitinophaga terraeKP01T(96.4 % 16S rRNA gene sequence similarity),Chitinophaga ginsengisegetisGsoil 040T(96.1 %),Chitinophaga arvensicolaIAM 12650T(96.1 %) andChitinophaga pinensisDSM 2588T(93.3 %). Strain YC6729Tcontained MK-7 as the major menaquinone and homospermidine as the major polyamine. The fatty acids of strain YC6729Twere iso-C15 : 0, C16 : 1ω5c, iso-C17 : 03-OH, C16 : 0, anteiso-C18 : 0and/or C18 : 2ω6,9c, iso-C15 : 02-OH and/or C16 : 1ω7c, C14 : 0, iso-C15 : 03-OH, iso-C15 : 1G, C18 : 1ω5c, iso-C15 : 1I and/or C13 : 03-OH, C13 : 02-OH, C16 : 03-OH and unknown fatty acid ECL 13.565. The polar lipid profile contained phosphatidylethanolamine, unknown aminolipids and unknown lipids. The total DNA G+C content of strain YC6729Twas 48.9 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed that strain YC6729Trepresents a novel species of the genusChitinophaga, for which the nameChitinophaga eiseniaesp. nov. is proposed. The type strain is YC6729T( = KACC 13774T = DSM 22224T).