Cohaesibacter marisflavi sp. nov., isolated from sediment of a seawater pond used for sea cucumber culture, and emended description of the genus Cohaesibacter

Abstract

A Gram-negative, catalase-negative, oxidase-positive, rod-shaped bacterium, strain DQHS21T, was isolated from sediment of a seawater pond used for sea cucumber culture at Jimo in Qingdao province on the east coast of China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DQHS21T belonged to the genus Cohaesibacter, sharing the highest sequence similarity (96.1 %) with Cohaesibacter gelatinilyticus CL-GR15T, while the similarity to other strains was below 93.0 %. The cellular fatty acids consisted mainly of C18 : 1ω7c (60.7 %), C18 : 0 (17.8 %), C16 : 0 (8.5 %) and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH; 6.0 %), which together accounted for 93 % of the total fatty acids. Ubiquinone 10 was the major quinone. The G+C content of the chromosomal DNA of strain DQHS21T was 55.2 mol%. The combined genotypic and phenotypic data showed that strain DQHS21T represents a novel species of the genus Cohaesibacter, for which the name Cohaesibacter marisflavi sp. nov. is proposed, with the type strain DQHS21T ( = CGMCC 1.9157T  = NCCB 100300T).