Affiliation:
1. Beijing Key Laboratory of Food Processing and Safety in Forestry, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China
2. College of Life Sciences, Langfang Normal University, Langfang 065000, PR China
3. Beijing Fairyland Environmental Technology Co., Ltd, Beijing 100085, PR China
Abstract
Strain CY1518T was isolated from an anaerobic fermentation liquid of food waste treatment plant in Beijing, PR China, and characterized to assess its taxonomy. Cells of CY1518T were Gram-stain-negative, oxidase-negative, catalase-positive and ellipsoidal. Growth occurred at 20–42 °C (optimum, 37 °C), pH 6.0–10.0 (optimum, pH 8) and with 0–6.0 % (w/v) NaCl (optimum, 1.5%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CY1518T belongs to the genus
Alcanivorax
, with the highest sequence similarity to
Alcanivorax pacificus
W11-5T (95.97 %), followed by
Alcanivorax indicus
SW127T (95.08%). The similarity between strain CY1518T and other strains of
Alcanivorax
was less than 95 %. The genomic DNA G+C content of strain CY1518T was 60.88 mol%. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between strain CY1518T and the closely related taxa
A. pacificus
W11-5T and
A. indicus
SW127T were 77.61, 78.03 and 21.2 % and 74.15, 70.02 and 19.3%, respectively. The strain was able to use d-serine, Tween 40 and some organic acid compounds for growth. The polar lipids comprised aminophospholipid, diphosphatidylglycerol, glycolipid, an unknown polar lipid, phosphatidylethanolamine, phosphatidylglycerol and phospholipid. The principal fatty acids (>5 %) were C19 : 0 cyclo ω8c (36.3%), C16 : 0 (32.3%), C12 : 0 3-OH (8.3%) and C12 : 0 (7.6%). Based on its phenotypic, genotypic and genomic characteristics, strain CY1518T represents a novel species in the genus
Alcanivorax
, for which the name Alcanivorax quisquiliarum sp. nov. is proposed. The type strain is CY1518T (=GDMCC 1.2918T=JCM 35120T).
Funder
Fundamental Research Funds for the Central Universities at Beijing Forestry University
University–Industry Collaborative Education Program
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology