Maribacter algicola sp. nov., isolated from a marine red alga, Porphyridium marinum, and transfer of Maripseudobacter aurantiacus Chen et al. 2017 to the genus Maribacter as Maribacter aurantiacus comb. nov.

Abstract

A Gram-stain-negative, strictly aerobic and moderately halophilic bacterial strain, designated strain PoM-212T, was isolated from a marine alga, Porphyridium marinum, in the Republic of Korea. Cells were catalase- and oxidase-positive rods with gliding motility without flagellum. Growth was observed at 20–40 °C (optimum, 30 °C) and pH 5.0–8.5 (pH 5.0–5.5) and in the presence of 0.5–5 % (w/v) NaCl (2–3 %). Strain PoM-212T contained iso-C15 : 1, iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) as major fatty acids and menaquinone-6 as the sole isoprenoid quinone. Phosphatidylethanolamine, three unidentified aminolipids and two unidentified polar lipids were detected as major polar lipids. The G+C content of the genomic DNA was 41.0 mol%. Strain PoM-212T was most closely related to Maribacter lutimaris KJ4T, Maripseudobacter aurantiacus CDA4T and Maribacter flavus C3T with 97.2, 97.2 and 97.0 % 16S rRNA gene sequence similarities, respectively, and it formed a distinct phylogenetic lineage from them within the genus Maribacter . Maripseudobacter aurantiacus formed a close phylogenetic lineage with Maribacter flavus with 99.7 % 16S rRNA gene sequence similarity, but their digital DNA–DNA hybridization value was 67.4 %. On the basis of its phenotypic, chemotaxonomic and molecular properties, strain PoM-212T represents a novel species of the genus Maribacter , for which the name Maribacter algicola sp. nov. is proposed. The type strain is PoM-212T (=KACC 19789T=JCM 32941T). The transfer of Maripseudobacter aurantiacus to the genus Maribacter as Maribacter aurantiacus comb. nov. is also proposed (type strain CDA4T=KCTC 52409T=MCCC 1K03210T).