Hymenobacter setariae sp. nov., isolated from the ubiquitous weedy grass Setaria viridis

Abstract

A Gram-stain-negative, short-rod, aerobic, non-motile, red to pink-pigmented bacterium, designated Fur1T, was isolated from the dry spikelet clusters of a plant called Setaria viridis near Dongguk University. Phylogenetic analysis conducted based on 16S rRNA gene sequences indicated that strain Fur1T belonged to the genus Hymenobacter of the family Hymenobacteraceae . The 16S rRNA gene of Fur1T showed highest sequence similarity to those of Hymenobacter metalli KACC 17381T (97.5 %) and Hymenobacter marinus KACC 19042T (97.1 %). Growth occurred at 4–37 °C (optimum, 25–28 °C), up to 1.0 % NaCl (optimum, 0 %) and pH 5.5–9.0 (optimum, pH 6.0–7.5). The major fatty acids of strain Fur1T were identified as iso-C15 : 0, C16 : 1  ω5c, anteiso-C15 : 0, summed feature 3 (comprising C16 : 1  ω7c and/or C16 : 1  ω6c) and summed feature 4 (comprising anteiso-C17 : 1B and/or iso-C17 : 1I) as the major cellular fatty acids. The predominant respiratory quinone was identified as MK-7. The polar lipids were phosphatidylethanolamine, five unidentified aminophospholipids, two unidentified phospholipids, one unidentified glycolipid and one unidentified polar lipid. The genomic DNA G+C content based on the draft genome sequence was 58.7 mol%. DNA–DNA relatedness between strain Fur1T and its closest relative was below 70 %. Characterization based on phylogenetic, chemotaxonomic and phenotypic analyses clearly indicated that strain Fur1T represents a novel species of the genus Hymenobacter , for which the name Hymenobacter setariae sp. nov. is proposed. The type strain is Fur1T (=KACC 19903T=NBRC=113691T).