Affiliation:
1. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, PR China
2. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China
Abstract
A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China. The strain grew at 25–45 °C and at pH 4.0–7.0, and was able to tolerate up to 50 mM Zn2+. The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4–0.8 % differences). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order
Ktedonobacterales
, showing <89.2 % sequence similarities to the recognized taxa of this order. The whole-genome based phylogenomic tree separated strain SCAWS-G2T from the recognized families within
Ktedonobacterales
. The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %. The strain can also be differentiated from the recognized families by a number of phenotypic characteristics. The polar lipids of SCAWS-G2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, seven unidentified glycolipids and one unidentified lipid. The peptidoglycan amino acids contained ornithine, glycine, glutamic acid and alanine, and the cell-wall sugars were mainly galactose and rhamnose. The major fatty acids were C16 : 1 2-OH, C16 : 0 and iso-C17 : 0. Based on all these data, we propose that strain SCAWS-G2T represents a novel genus and species, Ktedonosporobacter rubrisoli gen. nov., sp. nov., within the new family Ktedonosporobacteraceae fam. nov. of the order
Ktedonobacterales
. The type strain of Ktedonosporobacter rubrisoli is SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T).
Funder
National Natural Science Foundation of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
10 articles.
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