Affiliation:
1. Department of Biotechnology, Dong-A University, Busan 49315, Republic of Korea
2. Department of Microbiology, Pukyong National University, Busan 48513, Republic of Korea
Abstract
A novel Gram-stain-negative, facultatively anaerobic and rod-shaped bacterial strain, designated as DAU312T, was isolated from the sea water of the eastern coast of the Republic of Korea. Optimal growth was observed at 25 °C, pH 7.0–8.0 and with NaCl concentrations of 2.0 % (w/v). Catalase and oxidase activities were detected. On the basis of 16S rRNA gene sequences, strain DAU312T showed the highest similarity (99.2 %) to the type strain
Shewanella electrodiphila
MAR441T. The complete genome sequence of strain DAU312T contains 4 893 483 bp and 40.5 mol% G+C. Phylogenetic analyses based on 16S rRNA gene sequences and the up-to-date bacterial core genes showed that strain DAU312T,
S. electrodiphila
MAR441T and
S. olleyana
were all part of the same monophyletic clade. Their average nucleotide identity, digital DNA–DNA hybridization and two-way average amino acid identity values with each other and type strains of close
Shewanella
species were 83.4–77.5 %, 27.3–22.0 % and 89.8–81.2 %, respectively. The major cellular fatty acids (>10 %) were iso-C15 : 0, summed feature 3 (C16 : 1
ω7с and/or C16 : 1
ω6с) and C16 : 0. Phosphatidylethanolamine and phosphatidylglycerol were the main polar lipids. The respiratory quinones were Q-7, Q-8, MK-7 and MMK-7. Based on these polyphasic taxonomic findings, the name Shewanella goraebulensis sp. nov. is suggested for strain DAU312T, which is considered to represent a novel species of the genus
Shewanella
. The type strain is DAU312T (=KCTC 72427 T=JCM 35744T=KCCM 43478T).
Funder
Ministry of Science and ICT, South Korea
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology